Question

case study

Binding studies were carried out in which heparin was
immobilized on the surface of cuvettes. The pH
dependence of HPRG binding to heparin was
investigated and the results are shown in Figure 2.1.
a. How is the binding of HPRG to heparin
dependent on pH? Give structural reasons for the
binding dependence. The structure of heparin is
shown in Figure 2.2.
b. The same binding studies were carried out in
which HPRG was reacted with
diethylpyrocarbonate (DEPC), a compound that
specifically reacts with histidine residues. The
reaction is shown in Figure 2.3. Explain the
results.

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