Question

Calculate the p-NA concentration (mM) AND the number of micromoles (?mol) in each test tube

I am trying to do a lab write up but I am stuck on two things..

I know that I used 1.0 mM of the p-NA solution but where does that go into play here…

I do not know how to calculate the p-NA concentration (mM) AND the number of micromoles (µmol) in each tube.

And how do I convert the absorbance values into µmol p-NA.

This question is in part 1 and 3 right here…
http://www.liv.ac.uk/~agmclen/Medpracs/practical_3/methods_3.html

I know this question is all over the place, unfortunately so is my understanding of the topic.

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